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07 July 2022
Biorefinery and bioenergy sectors use plant cell wall as the main substrate. Therefore, the enzymatic degradation of plant cell wall becomes of great importance. Xylan is a major component of the plant cell wall, and the xylan present in hardwoods and grass families is frequently substituted with glucuronic acid units on the xylose backbone. In this study, two novel enzymes originating from Belliella baltica and Flavobacteriaceace bacterium from family GH115 were selected, expressed, purified, characterized, and tested on their capacity to debranch glucuronic acid from highly substituted polymeric xylan. The proteins’ melting temperature was determined with differential scanning fluorimetry (DSF), and the particle size through dynamic light scattering (DLS) and their structure was further visualized with cryoEM.
For more information, the full article is available here